Lebda Medical Journal

GC-MS analysis of Essential oil Components of the Libyan Thymus capitatus

2025-03-27T15:04:37+00:00

This study presents a comprehensive analysis of the essential oil components from Libyan Thymus capitatus utilizing gas chromatography-mass spectrometry (GC-MS) for identification and classification .The essential oil was extracted from samples collected in the Mesellata region of northwestern Libya. Among the identified compounds, monoterpene hydrocarbons were most prevalent, particularly Carvacrol, which constituted 56.27% of the total area. Other notable compounds included p-Cymene and γ-Terpinene. Oxygenated monoterpenes, led by Carvacrol, contributed significantly to the composition (69%), with Thymol, Linalool, and Terpinen-4-ol also being present. Additionally, Caryophyllene was identified as a significant Sesquiterpene hydrocarbon (3.57%), while Carvacryl acetate emerged as a notable oxygenated heterogeneous hydrocarbon, accounting for 1.81% of the composition. the findings highlight the potential health benefits and aromatic properties of these compounds, reinforcing the significance of Thymus capitatus essential oil as a source of natural bioactive compounds. This comprehensive analysis underscores the necessity for further exploration of this essential oil in food, cosmetic, and pharmaceutical applications

Impact of One Day Fundoscopy Workshop on Pediatrician's Fundus Examination Skills

2025-04-12T18:11:27+00:00

Abstract:

Background:

As physician's workloads increase, routine ophthalmoscopy may be dropped. And its number of frequency and competency in performing it are unknown.

Objective:

To assess the effectiveness of a daylong fundoscopy workshop and interactive lecture based education in improving pediatrician's fundus examination knowledge & skills.

Methods:

Pediatric doctors enrolled in Libyan board fellowship training program at Albayda Medical Center participated in this study. Pre and post-test comprising a pictorial quiz on the topic of presentation on which presentations & training workshop about fundus examination by direct ophthalmoscope were later delivered by 3 ophthalmology specialists were distributed to (n=32) participants in a one-day oriented workshop within a stipulated time period and their feedback was collected and statistically analyzed after the workshop was over.

Result:

A total 17 participants completed the quiz, varying between senior and junior pediatricians. All participants were included in the Libyan board fellowship training program. All 17 participants could not answer the pre-test pictorial quiz (0%), and after the tutorial 15(88.24%) participants were able to answer the quiz, this difference was statically significant (p = 0.000). A total of 29 participants completed the feedback questionnaire at the end of the workshop , the overall mean evaluation score by the participants was ≥ 4.1 out of 5. Participants satisfaction was (62.1 %) and non-satisfaction was (37.9%) (p =0.000).

Conclusions:

This study highlights the effectiveness of engaging teaching methods and fundoscopy hand on workshop to enhance fundoscopy examination and diagnostic skills in pediatrician. Also, it demonstrates the need for continuous professional development among healthcare professionals.

Key-words: pediatrician, fundoscopy, interactive lecture, skills, workshop

Real-World Effectiveness of Depot Medroxyprogesterone Acetate (DMPA): A Physician Survey on Failure Rates and Contributing Factors

2025-04-22T05:18:34+00:00

Background:

Depot medroxyprogesterone acetate (DMPA) is a widely used contraceptive, but real-world data on its performance remain limited.

Objective:

This study evaluates physician-reported experiences with DMPA, focusing on failure rates, side effects, and prescribing trends.

Methods:

A cross-sectional survey of 34 obstetrician-gynecologists in Libya assessed: (1) contraceptive failure rates and causes, (2) side effect prevalence, (3) patient adherence behaviors, and (4) provider preferences. Quantitative data were analyzed descriptively; qualitative responses were thematically coded.

Results:

Failure rates: 61.8% of physicians reported 0–1% failure (consistent with perfect use), while 29.4% observed 2–3% failure (attributed to non-compliance [32.4%] and improper administration [29.4%]).

Side effects: Irregular bleeding affected 52.9% of users; 38.2% experienced multiple concurrent side effects. Discontinuation due to side effects occurred in 38.2% of cases.

Prescribing patterns: 50% of providers preferentially recommended DMPA for specific patients (e.g., those needing estrogen-free options), while 35.3% favored alternatives due to adherence challenges.

Conclusions:

While DMPA demonstrates high efficacy in ideal use, real-world effectiveness is compromised by adherence barriers and side effects. Targeted interventions—including improved provider training, patient counseling, and reminder systems—could optimize outcomes. These findings support individualized contraceptive counseling and underscore the need for accessible alternatives in clinical practice.

Assessing the Applicability and Adherence to the CHADS2-VASc Score in Atrial Fibrillation

2025-03-10T18:51:56+00:00

Abstract:- Atrial fibrillation is the most important and common arrhythmia observed especially in the elderly population and it contributes very much in the morbidity of these patients due to mainly the significant risk of thromboembolic events.

In 2010 CHADS score was introduced followed by CHADS_VAS score in 2014 . since then it was added to most guidelines for the management of AF.

In this study we tried to look to whether we apply and adhere to these guidelines in zliten city.

Material and method:-

This study is a descriptive study using data collected from Zliten hospital Medical Dept., ICU and outpatient cases
Study population: a 100 AF diagnosed patients admitted to or followed in Zliten Hospital during 2015
Data collection: case study sheet prepared to collect data from the patients. The case study sheet contains variables needed to assess the use of mentioned guidelines

Conclusion:- in the study group we found a good adherence to the guidlines by our treating doctors

Does DYRK1A Regulate REST in Lung cancer?

2025-04-22T05:06:53+00:00

Purpose: The aims of this study were: (1) to screen a panel of lung cell lines for expression of DYRK1A protein (2) To investigate the correlation between DYRK1A and REST in normal lung and lung cancer. (3) To test whether DYRK1A depletion or chemical inhibition alters REST levels in lung cancer. (4) To determine if DYRK1A affects the transcription of the REST-target gene CHGB.
Method: Western blots were performed to detect the expression of DYRK1A and REST protein in extracts from normal lung cell lines, SCLC, carcinoid and NSCLC. siRNA silencing, we used 4 oligos for DYRK1A and tow positive control (2 oligos for REST) and non-targeting oligo as negative control. Harmine as specific DYRK1A inhibitor. MTS assay to tested cell viability and qRT-PCR to examine REST target gene transcript (CHGB).
Results: we found that DYRK1A expressed more in lung cancer than normal lung cell lines and there was a good correlation between DYRK1A and REST protein in normal lung cell lines. However, this correlation lost in some NSCLC cell lines. Also, DYRK1A depletion reduced REST level in H460 cell line. As well as the inhibition of DYRK1A in A549 reduce REST level. Finaly, CHGB responsed to DYRK1A inhibition in A549 and toDYRK1A depletion in H460 cell lines.