Does DYRK1A Regulate REST in Lung cancer?

Abstract

Purpose: The aims of this study were: (1) to screen a panel of lung cell lines for expression of DYRK1A protein (2) To investigate the correlation between DYRK1A and REST in normal lung and lung cancer. (3) To test whether DYRK1A depletion or chemical inhibition alters REST levels in lung cancer. (4) To determine if DYRK1A affects the transcription of the REST-target gene CHGB.
Method: Western blots were performed to detect the expression of DYRK1A and REST protein in extracts from normal lung cell lines, SCLC, carcinoid and NSCLC. siRNA silencing, we used 4 oligos for DYRK1A and tow positive control (2 oligos for REST) and non-targeting oligo as negative control. Harmine as specific DYRK1A inhibitor. MTS assay to tested cell viability and qRT-PCR to examine REST target gene transcript (CHGB).
Results: we found that DYRK1A expressed more in lung cancer than normal lung cell lines and there was a good correlation between DYRK1A and REST protein in normal lung cell lines. However, this correlation lost in some NSCLC cell lines. Also, DYRK1A depletion reduced REST level in H460 cell line. As well as the inhibition of DYRK1A in A549 reduce REST level. Finaly, CHGB responsed to DYRK1A inhibition in A549 and toDYRK1A depletion in H460 cell lines.