Isolation and Identification of Bacterial Pathogens from Hemodialysis Patients at Zliten Dialysis Center and Evaluation of Their Antibiotic Susceptibility Patterns

Abstract

A B S T R A C T

 Background: The most complication that Hemodialysis patient suffer from is get infected by microorganisms awhile they do the treatment these infections cause serious complications for dialysis patient especially catheter related bloodstream infection (CRBSIs). Aims: This study amid to isolate isolation and identification of bacterial pathogens associated with bloodstream infections and bacterial colonization of venous catheter sites from hemodialysis patients in Zliten City Libya., Then determine the major bacterial species and test their antimicrobial susceptibility patterns. Methods: A total of 124 clinical specimens were collected, comprising 62 catheter-site swab samples from peripheral venous catheter sites were conducted from hemodialysis patients and processed using standard microbiological procedures. The samples were cultured on appropriate selective and differential culture media and incubated under suitable conditions. The bacteria were examined based on colony morphology, microscopic characteristics, and Gram staining. Identification was performed using a series of conventional biochemical tests. Antimicrobial susceptibility testing was carried out to determine the susceptibility patterns of the isolated bacterial strains to commonly used antibiotics for hemodialysis patients.

Results: The results a total of 124 clinical specimens, comprising 62 catheter-site swab samples and 62 blood samples. 62 swab sample .93.55% (58 samples) showed bacterial growth, While 6.45% (4 sample) no bacterial growth. Among the 62 blood samples, showed result 22 bacterial growth. show the presence of several bacterial species associated with catheter sites and bloodstream infections. Antimicrobial susceptibility testing showed varying degrees of resistance among the bacterial isolates, with some strains exhibiting resistance to multiple classes of antibiotics. The results suggest the presence of multidrug-resistant bacterial strains and the potential risk of antimicrobial resistance within the hospital environment.

 Conclusion: catheter-related infections are the most concern for hemodialysis patients. Infection prevention and control practices, particularly appropriate catheter insertion, aseptic techniques, sterilization, and catheter care, is essential to reduce the risk of infection. In addition, antimicrobial therapy should be done by laboratory-based antimicrobial susceptibility testing. Continuous microbiological surveillance and rational antibiotic use are recommended to reduce the emergence and dissemination of antimicrobial-resistant bacteria among hemodialysis.